HPLC Analysis of Phenolic Compounds and Flavonoids with Overlapping ...
HPLC analysis. The HPLC analysis of the phenolic compounds and flavonoids was conducted using a Waters 2695 Alliance HPLC system (Waters Inc., Milford, CT, USA), equipped with a UVVis DAD. The separation was conducted using a Waters Sunfire TM C18 reversephase chromatography column, 250 mm length, mm width, and particle size 5 μm.